Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
نویسندگان
چکیده
منابع مشابه
In-vacuum long-wavelength macromolecular crystallography.
Structure solution based on the weak anomalous signal from native (protein and DNA) crystals is increasingly being attempted as part of synchrotron experiments. Maximizing the measurable anomalous signal by collecting diffraction data at longer wavelengths presents a series of technical challenges caused by the increased absorption of X-rays and larger diffraction angles. A new beamline at Diam...
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In the paper by Hussain et al. [(2015), J. Synchrotron Rad. 22, 465-468], Daniel Myatt is missing from the list of authors. The complete list of authors should be Rohanah Hussain, Kristian Benning, Daniel Myatt, Tamas Javorfi, Edoardo Longo, Timothy R. Rudd, Bill Pulford and Giuliano Siligardi.
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The B23 Circular Dichroism beamline at Diamond Light Source has been operational since 2009 and has seen visits from more than 200 user groups, who have generated large amounts of data. Based on the experience of overseeing the users' progress at B23, four key areas requiring the most assistance are identified: planning of experiments and note-keeping; designing titration experiments; processin...
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Determination of protein crystal structures requires that the phases are derived independently of the observed measurement of diffraction intensities. Many techniques have been developed to obtain phases, including heavy-atom substitution, molecular replacement and substitution during protein expression of the amino acid methionine with selenomethionine. Although the use of selenium-containing ...
متن کاملI12: the Joint Engineering, Environment and Processing (JEEP) beamline at Diamond Light Source
I12 is the Joint Engineering, Environmental and Processing (JEEP) beamline, constructed during Phase II of the Diamond Light Source. I12 is located on a short (5 m) straight section of the Diamond storage ring and uses a 4.2 T superconducting wiggler to provide polychromatic and monochromatic X-rays in the energy range 50-150 keV. The beam energy enables good penetration through large or dense ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2021
ISSN: 1940-087X
DOI: 10.3791/62364